The exclusion zone was set up around the farm last Thursday (June 7) after 22 chickens, three ducks and two peacocks were found to have the H7N2 strain of the virus.
It has not been confirmed that the Rainhill farm, on School Lane, housed the birds, but it is the epicentre of the restricted area which encompasses five farms, three local schools and a hotel, as well as part of the nearby M62 and forbids any person from moving poultry, other captive birds or any domestic animal, such as family pets, on to or off the premises without the approval of a veterinary inspector.
No one from the farm was available to comment.

The birds have been destroyed and have been linked to the recent outbreak, which has affected at least 22 people, in Chelford Market, Macclesfield, Cheshire.
They originally came from a small holding in Corwen, North Wales, where owners, Tony Williams and Barbara Cowling, alerted the authorities after their Rhode Island Red chickens had started to die.
Only two weeks ago, a St Helens couple, Mark Kinder and his girlfriend, Lauren Gedman, contracted the virus and were hospitalised. They have since been discharged and are recovering.

They have confirmed that they have nothing to do with the second outbreak but are concerned that they could catch it again and have been warned that a second infection could be a lot worse.
Mark said: "We have not been told anything about this outbreak, all we know is what we have read on teletext."
A Defra spokesperson said: "Tests have provided positive results for low pathogenic avian influenza in poultry on a non-commercial small holding near St Helens, Lancashire, England.

''Birds at the holding were purchased from the market held in Chelford on Monday, May 7, associated with the recent outbreak of H7N2 low pathogenic avian influenza in Conwy, North Wales."
Fred Landeg, Deputy Chief Veterinary Officer added: "The tests were carried out as part of the normal tracings activity which is under way following the outbreak in Wales.
"This is an extensive exercise, and this is the only premises to date with evidence of infection."
* Have you been affected by the bird flu outbreak? Contact our newsdesk on 01744 22285 or email sthelens.reporter@lancspublications.co.uk